In this study, we have developed a tooth regeneration model derived from mouse tooth germ cells based on tissue engineering. Lower first molars from DDY mice embryos at E 14 were used. Biodegradable Polyglycolate acid (PGA) scaffolds were fabricated, seeded with dissociated the first molars cells, and implanted into the subcapsular layer of kidney in an adult mouse where they allowed to develop over 3, 5, 7, and 14 days. We present histological analyses characterizing tissue-engineered tooth (TE-tooth). Histological analysis of 14 day implants tissue revealed mineralized enamel and dentin layer were apparent, of the kind of found in normally developing teeth.