To explore the influence of preservation by freezing on tissues of tooth germ, we examined changes in histological architectures and glycoconjugate expression detected by lectins, in tooth germs, with or without freezing, of postnatal 5 day rats. The results acquired were as follows: tissue architectures and cellular morphologies were similar between frozen and control groups. Stellate cells of enamel medulla and pulpal cells were, however, slightly atrophic at the control group. With lectin staining, there are no remarkable changes between frozen and control groups. From these results, we conclude that the influence of preservation by freezing was relatively small.