Isolation of bovine eosinophils by density gradient centrifugation
Copyright policy )Isolation of bovine eosinophils by density gradient centrifugation
SUMMARY A method for the isolation of an enriched population (> 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092. Viability was ≥ 95%, and the isolated eosinophils were found to be functional in chemokinetic and antibody-dependent cellular-cytotoxicity assays. This method represents a simple, rapid method for obtaining bovine eosinophils that can be used in a variety of assays of eosinophil function.
- Auburn University System United States
- Auburn University United States
Eosinophils, Chemotaxis, Leukocyte, Antibody-Dependent Cell Cytotoxicity, Centrifugation, Density Gradient, Zymosan, Animals, Cattle
Eosinophils, Chemotaxis, Leukocyte, Antibody-Dependent Cell Cytotoxicity, Centrifugation, Density Gradient, Zymosan, Animals, Cattle
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