Analisis 6 DNA Rekombinan dengan enzim EcoRI

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1997Publisher:Journal of Biological ResearchesJournal:Journal of Biological Researches, volume 3, pages 1-8 (issn: 0852-6834, eissn: 2337-389X,

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Authors: Ni Nyoman Tri Puspaningsih; null Akhmaloka; Afaf Baktir;

doi: 10.23869/bphjbr.3.1.19971

Analisis 6 DNA Rekombinan dengan enzim EcoRI

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Abstract

Amylase enzyme from Endomycopsis fibuligera capable to hidrolize starch into glucose. Insertion of amylase gene from Endomycopsis fibuligera into yeast (Saccharomyces cereviceae) will be able to increase the function of yeast (S.cereviceae) to digest more cheaper substrate, like starch. Before cloning in yeast, recombinant DNA will be made and analyzed in Escherichia coli strain DH5Î±. The result showed that the sixth transformant consist recombinant DNA that were sensitive to tetracycline medium. Analysis by EcoR1 digestion showed that the size of insertion fragment into Ycp 50 vector are around 0.3 untill 16 kb.

Keywords

Amylase gene, QH301-705.5, Science, Q, EcoR1 digestion, Biology (General), Cloning

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