This paper represents the second of a monographic series on the trematode family Brachycoeliidae Johnston, 1912. In Part I (Cheng, 1957) the author referred to this family of flukes as members of the subfamily Brachycoeliinae Looss, 1899; however, at that time he stated that he agreed with Fuhrmann (1928) ". . . in retaining Brachycoeliinae as a subfamily under Dicrocoeliidae until additional evidences can indicate differently." Since the appearance of Part I, the life history of Brachycoelium obesum Nicoll, 1914 has been satisfactorily established and that of B. salamandrae (Frolich, 1789) has been partially established. The information obtained from these life cycles strongly suggest that the subfamily Brachycoeliinae should be granted independent status as the family Brachycoeliidae as suggested by Johnston (1912), since the life histories of the Brachycoelium spp. indicate the presence of only one intermediate host (the entire life histories will be published at a later date) which differs sharply from the life cycles of either the Dicrocoeliidae or the Plagiorchiidae where two intermediate hosts exist. Since Rankin's (1944) description of the life cycle of Glypthelmins quieta contained only one intermediate host, this further suggests the close relationship between Glypthelmins and Brachycoelium, hence the author has chosen to assign the genera covered in Part I and those included in Part II as members of the independent family Brachycoeliidae. Inasmuch as the members of the genus Brachycoelium are different from those of Glypthelmins and Margeana (see Cheng, 1957) the author has chosen to retain the subfamily Brachycoeliinae Looss, 1899 under Brachycoeliidae to include Brachycoelium and has erected the Glypthelminae n. subfam., also unider Brachycoeliidae, to include Glypthelmins, Margeana and Reynoldstrema. This present paper consists of a revision of Glypthelmins (Stafford, 1900) Stafford, 1905, the re-establishment of Margeana Cort, 1919 as a valid genus, and the description of Reynoldstrema n. gen. The descriptions of the various species of the genera considered, have been restricted to the essentials for the sake of brevity. The original type drawings have been included to aid in the diagnosis of specimens. The genera Glypthelmins and Margeana are extremely closely