An electrophoretic comparison was made between Anolis carolinensis from Texas and Georgia and Anolis porcatus from Cuba. At five of 35 presumptive gene loci the Cuban population may be distinguished from the North American population. The Nei genetic distance (o) estimate between the Cuban and North American populations is 0.165 which is slightly above interpopulation, intraspecific distances that have been measured in other lizards. A molecular clock prediction of a Pliocene date of colonization of North America by a Cuban porcatus stock is congruent with a divergence time estimate based on immunological data.