The authors present results of a planned prospective study of infections with Chlamydia trachomatis in women. Direct immunofluorescence test (DIF) for proving Chlamydia trachomatis antigens in endocervical smear (by means of monoclonal antibodies), ELISA test for proving IgG antibodies against Chlamydia trachomatis in sera and RVK method for proving antibodies against group Chlamydia-antigen were applied to test 288 women (aged 19-67), previously thoroughly anamnestically and clinically examined. Chlamydia trachomatis infection was found in 29.51% of the cases, equally distributed in all age groups. The authors analyzed frequency of Chlamydia positive findings according to clinical diagnoses, parity, relevant data from gynecological history, as well as results of vaginal secretion tests and Papanicolaou smear findings.