The adsorption of T-2 toxin by the natural smectite mineral-hectorite at pH 3.0, 7.0 and 9.0 was investigated. The results of T-2 toxin adsorption on hectorite showed that the T-2 adsorption capacity decreased with increasing concentration of adsorbent in the suspension for all the investigated pH values. From the adsorption isotherms, an increase in T-2 toxin adsorption with increasing initial T-2 toxin concentration was observed for all the investigated pH values. The T-2 toxin adsorption by hectorite followed a non-linear (Langmuir) type of isotherm at pH 3.0, 7.0 and 9.0, with correlation coefficients (r2) of 0.943 at pH 3.0, 0.919 at pH 7.0 and 0.939 at pH 9.0. The estimated maximum T-2 toxin adsorption by hectorite based on the Langmuir fit to the data (9.178 mg/g at pH 3.0, 9.930 mg/g at pH 7.0, and 19.341 mg/g at pH 9.0), indicated that the adsorption of T-2 toxin by hectorite is pH dependent. The obtained data suggest the existence of specific active sites in hectorite onto which the T-2 toxin is adsorbed.