In this paper, we investigated the enzymatic treatment of polyester fabric. The results show that enzymatic treatment with different Upases causes adequate effects, especially, referring to water penetration, absorption and the mechanical parameters of the processed fabric (strength, elongation, wear resistance). The results of scanning electron microscopy contributed to the structural and morphological understanding of the polyester fiber surface in the treated regime. After enzyme treatment, some changes on the fiber surface were noticed. These results confirmed that the enzymes influenced the surface of the polyester fibers. The process probably did not cause major damage of the fiber surface or major reorganization of the surface layers of the polyester fibers, so therefore their mechanical characteristics were satisfactory.