Chlamydiosis is a contagious disease of birds, mammals, reptiles and humans. So far it was demonstrated in 469 species of birds and among them, turkeys are the most susceptible domestic poultry species. The disease appears in epizootic form in intensive turkey farming. Since commercial poultry rearing is under-developed in Bosnia and Herzegovina, our investigation was based on extensively reared turkeys. Cloacal and oropharyngeal swabs were taken from 26 birds and infection was proven by common chlamydial LPS antigen detection tests (IDEIA and CW). We have used rRT-PCR technique targeting chlamydial ompA gene region in order to prove Chlamydia species. Five birds, (19.2%) were found positive as judged by IDEIA and CW tests. Among them one was positive Cp. psittaci speciesspecific rRT-PCR, ompA gene.