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Article . 2010 . Peer-reviewed
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PRODUÇÃO DE INVERTASE POR FERMENTAÇÃO EM ESTADO SÓLIDO A PARTIR DE FARELO DE SOJA

Authors: Lexandra Novaki; Marina K. Kadowaki; Salah D. M. Hasan; Diandra Andrade;

PRODUÇÃO DE INVERTASE POR FERMENTAÇÃO EM ESTADO SÓLIDO A PARTIR DE FARELO DE SOJA

Abstract

A invertase é responsável pela hidrólise da sacarose na formação de açúcar invertido e é utilizada em indústrias alimentícias e farmacêuticas. Neste trabalho objetivou-se a produção da invertase por fermentação em estado sólido (FES) do farelo de soja por Aspergillus casiellus. Obteve-se atividade no extrato bruto de 1029,75 U.mg-1 após 72h de fermentação. A recuperação por precipitação com sulfato de amônio esolventes foi menos efi ciente que com o choque térmico. Análises de SDS-PAGE indicaram massa molecular da invertase em torno de 25,5 kDa e a mesma manteve as condições ótimas em pH 4,0 a 70ºC com 50% de atividade residual. Comprovou-se que o uso de resíduo agroindustrial para obtenção da invertase é uma alternativa importante visando o desenvolvimento de tecnologias limpas, convertendo o resíduo em um produto de maior valor agregado.

  • BIP!
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    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    9
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
9
Average
Average
Average
gold