Objective: To evaluate effect of ethyl acetate fraction isolated from Vernonia cinerea (L) (EAVC) in acute lung inflammation in rats. Methodology: Rats were pretreated with vehicle (CMC 0.5%), EAVC(25, 50 & 100 mg/kg po), roflumilast (1mg/kg po), once a day for 3 consecutive days. 2hrs after the last treatment the rats were intratracheally injected with lipopolysaccharide (LPS), (1mg/kg) while control group received saline(1ml/kg) under anesthesia. After 4hrs of intratracheal LPS injection, animals were sacrificed and BALF was collected. Lungs were perfused, dissected out and used for biochemical estimations and histological examination. Results: EAVC (50 & 100 mg/kg po) significantly (p < 0.05) inhibited MPO, total protein, albumin, total cells, neutrophils and histamine content in BALF indicating suppression of lung permeability. Similarly, MOP, catalase enzyme activity in lung tissue homogenate were reduced as compared to positive control group. Histological evaluation revealed marked reduction in perivascular infiltration and edema in drug treated animals. Conclusion: EAVC (50 & 100 mg/kg po) exhibited protective effect in LPS induced ALI in rats by inhibiting lung permeability and inflammation.