This study deals with the isolation and characterization of parasitic infection in Channa punctata collected from different water bodies in Jessore, Bangladesh. Skins, gills, intestine, kidney, liver were observed for finding parasites. In total thirty parasites were identified under five species from fifty experimental fish. Different types of parasites such as cestodes, platyhelminth, nematode, acanthocephalan and liver fluke were found from experimental fish. The high incidence of parasite infection in Channa punctatus was found in the river, lake or beel area compares with the farmed fish.