Biosafety Level 4 facilities have been established in our country to promote research and development and national capacity of diagnostic test systems for infectious diseases, including highly pathogenic viruses. In particular, the operation of a suit-type laboratory requiring positive pressure protective suits is a first for our country, and it is necessary to establish unique safety management technology. With the support of the Research Program on Emerging and Re-emerging Infectious Diseases of the Japan Agency for Medical Research and Development (AMED), we conducted research entitled "Study of Practical Biorisk Management in the Microbiological Containment Laboratories" from 2021 to 2023, with the aim of improving biosafety management technology in Japan. We have summarized our research project and its results obtained for the future direction of biorisk management research in Japan.