Xanthomonas citri subsp citri (Xcc) is responsible for one of the most destructive diseases of citrus fruits, Asiatic citrus canker. Based on the bacterial host range, the pathogenic forms, A, A*, and Aw have been identified. A*-type strains are not well characterized and they have been reported to differ from other A-type strains on the basis of biochemical and genetic characteristics. In this study, we attempted to develop a medium to mimic in planta conditions for inducing protein secretion of the A*-type strain-NIGEB-088. We also attempted to achieve efficient extraction of Xcc A*-type extracellular proteins, the key mediators of plant-bacterium interaction. Results obtained by this study demonstrate that the m3 (mMM1) can induce Xcc A*-type strains to secret extracellular proteins in amounts detectable by two dimensional gel electrophoresis (2-DE). We also show that Pyrogallol red-molybdate methanol (PRMM) is more effective for precipitation of Xcc A*-type secreted proteins than other assessed procedures of protein precipitation, i. e. acetone, TCA, and sodium deoxycholate