Proteomic analysis of phosphoproteins and its application to myelin basic proteins.

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2005Publisher:Japanese Electrophoresis SocietyJournal:SEIBUTSU BUTSURI KAGAKU, volume 49, pages 73-81 (issn: 0031-9082, eissn: 1349-9785,

Copyright policy )

Authors: Kyoichi Akiyama; Nobuko Shimada; Narimichi Kimura; Tosifusa Toda;

doi: 10.2198/sbk.49.73

Proteomic analysis of phosphoproteins and its application to myelin basic proteins.

- Summary
- Metrics

Abstract

従来タンパク質のリン酸化は, ラジオアイソトープ標識法やウエスタンブロット法を用いて研究されてきたが, 我々は質量分析法による高感度分析法の有効性について基礎的な検討を行った. まずリン酸化部位が既知の標準ペプチドやカゼインを用いて, Post-source decay モードによるMALDI-TOF質量分析を行い, セリンおよびトレオニンリン酸化ペプチド検出における有効性を確認した. しかしチロシンリン酸化ペプチドには, 必ずしも有効ではないこともわかった. 検出感度を上げるために, 市販のリン酸化ペプチドおよびリン酸化タンパク質濃縮キットの使用方法の最適化を行い, 有効性を確認した. さらに我々はこの方法をラットミエリンのミエリン塩基性タンパク質の分析に応用し, リン酸化ペプチドの高感度検出と, 新しいリン酸化部位の特定に成功した.

Related Organizations

Tokyo Metropolitan Institute of Gerontology
Japan

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	0
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average

Found an issue? Give us feedback

0

Average

bronze