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Biological Sciences in Space
Article . 2001 . Peer-reviewed
Data sources: Crossref
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Microflora Investigation Experiment

Authors: K, Harada;

Microflora Investigation Experiment

Abstract

Many microorganisms were isolated from condensed water, wiping and scratching of cabin wall and air sampler in Russian space station Mir by Russian astronauts Lazutkin et al. in February 1997 as part of NASDA "First MIR Utilization Space Experiment (JMIR)". For example, there were about 2 x 10(6) cells/ml in condensed water sample No. 1 isolated from the transfer-docking compartment of Crystal module. We tried the colony isolation, pure culturing and identification from these sampled microorganisms. After the bacteria separated from filamentous fungi and yeasts were observed using the phase contrast optical microscopy and gram stain method, twenty-one kinds of biochemical characters, e.g., oxidase test, activity of beta-galactosidase and fermentation of glucose etc., were investigated on the isolated bacteria. Then, we found Serratia liquefaciens and Yersinia enterocolitica and the chemoheterotroph Pseudomonadaceae, Stenotrophomonas maltophila. Furthermore, using ultraviolet (UV) lamp we tried the isolation of radioresistant bacteria, we found the radioresistant Sphingomonas paucimobilis (90.8% identification) by comparing with radioresistant Escherichia coli B/r strain. Considering Mir environment under cosmic radiation, this radioresistant S. paucimobilis might be the mutant from radiosensitive one. Following papers were published [see text].

Keywords

Bacteria, Weightlessness, Yeasts, Colony Count, Microbial, Environmental Microbiology, Fungi, Space Flight, Spacecraft, Radiation Tolerance

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
2
Average
Average
Average
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