
In this paper, new multiphoton microscopy using CARS (coherent anti-Stokes Raman scattering) spectroscopy is described. CARS microscopy has the features of non-staining molecular mapping by molecular vibration imaging and three-dimensional resolution by multiphoton process. A picosecond tunable laser and suitable optical filters provide the CARS imaging in the fingerprint region, and multi-focus excitation using a rotatory-microlens array enables the multi-spectral imaging.
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