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Article . 2015 . Peer-reviewed
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BIO-PROTOCOL
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Genetic Transformation of Candida glabrata by Electroporation

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 01 Jan 2015 English Publisher:Bio-Protocol, LLCJournal:BIO-PROTOCOL, volume 5 (issn: 2331-8325, Copyright policy )Funded by:EC | FUNGITECT
Authors: Fabian Istel; Tobias Schwarzm�ller; Michael Tscherner; Karl Kuchler;

doi: 10.21769/bioprotoc.1528

pmid: 27774499

pmc: PMC5074361

Genetic Transformation of Candida glabrata by Electroporation

Abstract

Here, we report a method for the transformation by electroporation of the human fungal pathogen Candida glabrata (C. glabrata). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using the C. glabrata background recipient strain ATCC2001 (Schwarzmüller et al., 2014).

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QH301-705.5, Biology (General)

  • BIP!
    Impact byBIP!
    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 6
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
6
Average
Average
Average
gold
Funded by
Related to Research communities
Neuroscience