Influence of cell dissociation procedures on the tumorigenicity of Simian Virus 40 transformed fibroblasts
Influence of cell dissociation procedures on the tumorigenicity of Simian Virus 40 transformed fibroblasts
Mouse fibroblasts transformed by Simian Virus 40 (SV40) were examined for tumor forming ability in syngeneic BALB/c mice following dissociation from tissue culture dishes by two procedures. A significantly greater in vivo proliferative capacity was observed for cells dissociated by the tryspin-EDTA procedure, with the injected cell dose for tumor production in 50 percent of recipient mice (the TPD$sub 50$) being 16-fold lower than the TPD$sub 50$ for cells dissociated by the EDTA procedure. Host immunosuppression with 300 rad whole-body $gamma$ irradiation led to a significant 7-fold decrease in the TPD$sub 50$ for cells dissociated by the EDTA procedure, while no significant decrease in TPD$sub 50$ was observed for cells dissociated by the tryspin-EDTA procedure. (auth)
- University of North Texas United States
- University of Utah United States
- University of California, Berkeley United States
Gamma Radiation, *Mice-- Biological Radiation Effects, *Oncogenic Viruses-- Biological Effects, Carcinogenesis, 610, Fibroblasts, 560152* --Biomedical Sciences, N48520* --Life Sciences--Radiation Effects On Animals-- Vertebrates, *Immunosuppression-- Radioinduction, Morphological Changes, Applied Studies--Radiation Effects--Radiation Effects On Animals--Vertebrates, Cytology, Graft-Host Reaction, *Tumor Cells-- Cell Proliferation
Gamma Radiation, *Mice-- Biological Radiation Effects, *Oncogenic Viruses-- Biological Effects, Carcinogenesis, 610, Fibroblasts, 560152* --Biomedical Sciences, N48520* --Life Sciences--Radiation Effects On Animals-- Vertebrates, *Immunosuppression-- Radioinduction, Morphological Changes, Applied Studies--Radiation Effects--Radiation Effects On Animals--Vertebrates, Cytology, Graft-Host Reaction, *Tumor Cells-- Cell Proliferation
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