This paper presents a thermal analysis and methodology of thermal parameters identification of thermal multilayer structures using thermographic measurements after dynamic heating (or cooling) the investigated objects. During the thermal transient process, thermal images are recorded, with the highest possible frame rate of the camera. Then, temperature and power signals are transformed into the frequency domain, to get finally the thermal time constants distribution corresponding to the thermal parameters of the structure. A brief description of TOI program developed in MATLAB environment as well as the exemplary identifications of 2 different thermal objects are shown.