The worrying Newcastle disease is endemic in Egypt with outbreaks occurring from one time toanother causing dramatic economic losses in poultry flocks. The current study was a trial to isolateand identify NDV from poultry flocks in Egypt during 2015 and 2016. The NDV was isolated in theallantois cavity of specific pathogen free embryonated chicken eggs (SPF-ECE). Thehemagglutination positive isolates were identified as NDV by Hemagglutination inhibition test (HI)and genetically by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Estimating thevirulence of the isolated NDV by partial F gene sequencing and intra-cerebral pathogenicity index(ICPI)calculation that was found to be (1.51). Titer of the propagated NDV isolate was 10 log2HAand by ECE inoculation was 109.7 EID50/ml. Our results indicated that the new isolate is virulent andgenetically related to genotype VIId NDV which emphasize the importance of NDV surveillance forimproving strategies for disease control.