The static transmission error is one of the key incentives of gear dynamics and addressed by many scholars. However, the traditional calculation method of static transmission errors of spur gears does not take into account the influence of thermo-elastic coupling caused by the gear temperature field, and it limits the accuracy of the dynamic characteristic analysis. Thus, in this study, the calculation method of static transmission errors associated with thermo-elastic coupling is proposed. Furthermore, the differences between static transmission errors associated with thermo-elastic coupling contacts and traditional method of gear is discussed. The study is helpful to improve the accuracy of dynamic analysis of gear transmission system.