To evaluate the usefulness of the newly designed 25-gauge (25-G) microcannula for transconjunctival sutureless vitrectomy (TSV).25-G TSV was performed on 20 consecutive eyes with the newly designed 25-G microcannulas. We studied usefulness, intraoperative and postoperative complications in these cases.No complications were recognized during surgery. Postoperative complications included blebs in five eyes, vitreous hemorrhage in one eye, high intraocular pressure (more than 22 mmHg) in one eye and a retinal tear in one eye. No eyes with low intraocular pressure (less than 10 mmHg) were noted.This microcannula for 25-G vitrectomy is thought to be very useful and effective for performing 25-G vitrectomy safely.