Phylogenetic relationships among the genera of the filarioid worm of subfamily Dirofilariinae were analysed based on cladistic. Fourteen characters among Macacanema, Edesonfilaria, Dirofilaria, Loaina, Pelecitus, Foleyella, Loa and Bostrichodera were compared resulting in a consensus phylogenetic tree. The last six genera form a group with at least one synapomorphy, and Foleyella, Loaina and Pelecitus are the most derived among the subfamily. Edesonfilaria and Macacanema most probably belong to a subfamily other than Dirofilariinae.