A Simple and Reliable Method for Determination of Optimum PH in Coupled Enzyme Assays
Copyright policy )A Simple and Reliable Method for Determination of Optimum PH in Coupled Enzyme Assays
Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kinase/lactate dehydrogenase coupled enzyme assay. This simple and reliable method can be broadly adapted to determine the pH optimum for various enzymes that are assayed using a coupled enzyme assay.
- Dartmouth College United States
- University of Alabama at Birmingham United States
- Birmingham–Southern College United States
coupled enzyme assay, Cryptosporidium parvum, L-Lactate Dehydrogenase, pH optimization, QH301-705.5, Plasmodium falciparum, Pyruvate Kinase, Temperature, Reproducibility of Results, lactate dehydrogenase, Hydrogen-Ion Concentration, enzyme kinetics, Enzyme Stability, Biology (General), Enzyme Assays
coupled enzyme assay, Cryptosporidium parvum, L-Lactate Dehydrogenase, pH optimization, QH301-705.5, Plasmodium falciparum, Pyruvate Kinase, Temperature, Reproducibility of Results, lactate dehydrogenase, Hydrogen-Ion Concentration, enzyme kinetics, Enzyme Stability, Biology (General), Enzyme Assays
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).12 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!