Site-Directed Mutagenesis of Large Plasmids

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 1998 English Publisher:Informa UK LimitedJournal:BioTechniques, volume 25, pages 1,014-1,019 (issn: 0736-6205, eissn: 1940-9818,

Copyright policy )

Authors: Susan A. Nadin-Davis; Shirley C. Chang;

doi: 10.2144/98256st04

pmid: 9863055

Site-Directed Mutagenesis of Large Plasmids

- Summary
- Subjects
- Metrics

Abstract

A protocol combining recombination PCR and long-distance PCR is demonstrated to be highly accurate and rapid for site-directed mutagenesis of large (> 10 kb) plasmids. Application of this protocol to the generation of mutant rabies virus glycoproteins expressed by the baculovirus/insect cell system illustrates the usefulness of this approach in facilitating structure-function relationships in this important eukaryotic expression system.

Related Organizations

Canadian Food Inspection Agency
Canada

Keywords

Gene Expression Regulation, Viral, Recombination, Genetic, Insecta, Genes, Viral, QH301-705.5, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Genetic Vectors, Molecular Weight, Transformation, Genetic, Rabies virus, Mutagenesis, Site-Directed, Animals, Genetic Testing, Biology (General), Baculoviridae, Cells, Cultured, DNA Primers, Glycoproteins, Plasmids

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	6
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average