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BioTechniques
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BioTechniques
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BioTechniques
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BioTechniques
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https://dx.doi.org/10.2144/053...
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Semiautomatic Detection of DNA Methylation at CpG Islands

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2005 English Publisher:Informa UK LimitedJournal:BioTechniques, volume 38, pages 354-358 (issn: 0736-6205, eissn: 1940-9818, Copyright policy )
Authors: Kyeong-Man Hong; Sei-Hoon Yang; Mingzhou Guo; James G. Herman; Jin Jen;

doi: 10.2144/05383bm02

pmid: 15786801

Semiautomatic Detection of DNA Methylation at CpG Islands

Abstract

or the unmethylated sequences using the SNaPshot™ kit (Applied Biosystems, Foster City, CA, USA). This kit uses the single base extension (SBE) principle to detect variations of single nucleotide polymorphisms. The extended products were then analyzed on an ABI Prism ® 3100 Genetic Analyzer (Applied Biosystems), and the status of DNA methylation was determined based on the relative peak heights of the extended products generated from methylated and unmethylated templates. In this report, we used the detection

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Keywords

Lung Neoplasms, QH301-705.5, DNA Mutational Analysis, DNA, Sequence Analysis, DNA, DNA Methylation, Polymerase Chain Reaction, Pattern Recognition, Automated, Biomarkers, Tumor, Humans, CpG Islands, Biology (General), Sequence Alignment, Cyclin-Dependent Kinase Inhibitor p16

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    popularity
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
5
Average
Average
Top 10%
gold