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BioTechniques
Article . 2004 . Peer-reviewed
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BioTechniques
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BioTechniques
Article . 2004
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BioTechniques
Article . 2004
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Comparison of different probe-level analysis techniques for oligonucleotide microarrays

Authors: Samantha Rodriguez; Anneke Kuehler; Frederic Grau; David McKinnon; Barbara Rosati;

Comparison of different probe-level analysis techniques for oligonucleotide microarrays

Abstract

Three different software packages for the probe-level analysis of high-density oligonucleotide microarray data were compared using an experiment-derived data set that was validated using real-time PCR. The efficiency with which these three programs could identify true positives in this data set was assessed. In addition, estimates of false-positive and false-negative rates were determined. The performance of the programs using very small data sets was also compared, and recommendations for use are suggested.

Related Organizations
Keywords

QH301-705.5, Gene Expression Profiling, Myocardium, Polymerase Chain Reaction, Rats, Animals, False Positive Reactions, Biology (General), Oligonucleotide Probes, False Negative Reactions, Oligonucleotide Array Sequence Analysis

  • BIP!
    Impact byBIP!
    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    18
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 10%
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
18
Average
Average
Top 10%
gold