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BioTechniques
Article . 2001 . Peer-reviewed
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BioTechniques
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BioTechniques
Article . 2001
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BioTechniques
Article . 2001
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https://dx.doi.org/10.2144/013...
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Genetic Transformation of Yeast

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 2001 English Publisher:Informa UK LimitedJournal:BioTechniques, volume 30, pages 816-831 (issn: 0736-6205, eissn: 1940-9818, Copyright policy )
Authors: R. Daniel Gietz; Robin A. Woods;

doi: 10.2144/01304rv02

pmid: 11314265

Genetic Transformation of Yeast

Abstract

Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the transformation of Saccharomyces cerevisiae. The application of protocols developed for S. cerevisiae to other important yeast species is discussed. The protocols for transformation by spheroplasting, LiAc/ssDNA/PEG, and electroporation are compared, and possible mechanisms for transformation are discussed.

Related Organizations
Keywords

Transformation, Genetic, QH301-705.5, Gene Transfer Techniques, Saccharomyces cerevisiae, Biology (General)

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 180
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 1%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 1%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 10%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
180
Top 1%
Top 1%
Top 10%
gold