This retrospective study used data on bovine abortion submissions from 5 U.S. veterinary diagnostic laboratories (VDLs) from 2000-2011. Objectives of this study were to describe the prevalence and patterns of BHV-1-associated abortion in submissions at VDLs. There were three specific aims: 1) examine the proportion of BHV-1-associated abortions with the introduction of new diagnostic assays, 2) evaluate the agreement of the histopathology report of the abortion submissions and the result of the assay used, and 3) evaluate the association between farm history of vaccination against BHV-1 and BHV-1-positive abortion submissions.