Chicken agonistic behavior, a type of social behavior related to threatening and fighting, is among the most serious problems in the poultry industry. However, due to luck of effective models for investigating the brain mechanisms of the behavior, no effective measures have been taken. This study, therefore, aimed to select the behavioral tests available for monitoring chicken agonistic behavior. Two behavioral tests, resident-intruder (R-I) test and social interaction (SI) test, were performed for 10 minutes in 10 pairs of male layer chicks at 8, 12, 16, 20, and 24 days of age, and total agonistic frequencies (TAF: Sum of the frequencies of agonistic displays like pecking, biting, kicking, threatening, and leaping) and latency (the period of time from the beginning of the behavioral test to the occurrence of the first agonistic behavior) were measured as indices of agonistic behavior. Two-way repeated measures ANOVA revealed significant differences in TAF and latency between aggressors and opponents in both the behavioral tests. In the R-I test, the TAF of aggressors significantly increased from 8 to 20 days of age, and the latency significantly decreased from 8 to 24 days of age. In the SI test, however, the TAF of aggressors significantly increased and the latency significantly decreased only from 16 to 20 days of age. When the criterion of high agonistic behavior was defined as the TAF, where aggressors showed more than 30 times of TAF and the opponents did less than one-third TAF of aggressors, the aggression establishment rate (AER), which is equal to the number of aggressors showing high agonistic behavior per total behavioral trials, was significantly higher in the R-I test than in the SI test. These results suggest that the R-I test, rather than the SI test, is an effective tool for monitoring agonistic behavior of layer chicks.