Nanocarriers have attracted significant interest due to their versatile functionalities that support advanced drug delivery applications. In this context, novel copolymers based on ethylene brassylate and glycidyl methacrylate were synthesized and investigated as precursors for nanoparticle formation with potential biomedical applications. The copolymer structure was confirmed by spectroscopic techniques, and their physicochemical properties were correlated with their self-assembly behavior in aqueous media. The resulting nanoparticles exhibited sizes within the nanometric range, good colloidal stability, and relatively narrow size distributions, indicating a reproducible formulation. These findings highlight the potential of the developed system for the design of multifunctional nanocarriers suitable for the controlled release of therapeutic agents.