European canker, caused by the fungus Neonectria ditissima, is one of the most damaging diseases affecting apple cultivation, causing necrosis in branches, trunks, and fruit, which severely compromises the productivity and longevity of orchards. The difficulty in managing the disease stems largely from the long latency period of the pathogen until visible symptoms appear. In this context, the present study aimed to develop and validate a rapid and sensitive molecular method for the detection of N. ditissima in apple tree tissues using the loop-mediated isothermal amplification (LAMP) technique. Specific oligonucleotides were designed for the ITS region of the fungus' ribosomal DNA using Primer Explorer v5 software. Fungal and plant samples underwent DNA extraction using the phenol: chloroform and CTAB methods, respectively. The technique was optimized for a temperature of 66 °C, with detection in 60 minutes and visualization by fluorescence under UV light. The primers developed showed high specificity, exhibiting no cross-reactions with other fungi associated with apple trees, and allowed a detection limit of 20 fg of DNA. Tests performed with symptomatic samples confirmed the robustness of the method. The results demonstrate that the LAMP test is a promising alternative, quick to perform and highly sensitive, capable of aiding in the early diagnosis of European canker and contributing to more efficient monitoring and phytosanitary management strategies in apple orchards.