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Veterinary and Animal Science
Article . 2026 . Peer-reviewed
License: CC BY NC ND
Data sources: Crossref
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Veterinary and Animal Science
Article . 2026
Data sources: Europe PubMed Central
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PubMed Central
Article . 2026
License: CC BY NC ND
Data sources: PubMed Central
https://doi.org/10.2139/ssrn.5...
Article . 2025 . Peer-reviewed
Data sources: Crossref
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Comparison of two DNA extraction methods for the detection of zoonotic protozoan pathogens in oysters and seawater

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2025Publisher:Elsevier BVJournal:Veterinary and Animal Science, volume 32, page 100,592 (issn: 2451-943X, Copyright policy )
Authors: Veronica Rodriguez Fernandez; Minji Kim; Karen Shapiro;

doi: 10.2139/ssrn.5519736 , 10.1016/j.vas.2026.100592

pmid: 41737898

pmc: PMC12927077

Comparison of two DNA extraction methods for the detection of zoonotic protozoan pathogens in oysters and seawater

Abstract

We compared freeze-thaw and bead beating methods for extracting DNA from parasites in oysters and seawater. Bead beating performed better for extracting Cryptosporidium, Giardia, and Toxoplasma in seawater, while freeze-thaw yielded comparable or better detection for Cryptosporidium and Giardia in oysters. This comparison highlights relative strengths and limitations of these methods.

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    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 1
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
1
Average
Average
Average
Green
gold