Artificial light at night (ALAN) can disrupt the natural behaviour, physiology, and circadian rhythms of organisms exposed to it, and therefore presents a significant and widespread ecological concern. ALAN typically comprises a wide range of wavelengths, and different wavelengths have different effects on circadian clocks. In the animals investigated thus far, short and middle wavelengths are intensely involved in synchronisation and entrainment, but we still have a poor understanding of how different wavelengths might affect behaviour when animals are exposed to ALAN, in particular whether some wavelengths are disproportionally detrimental. This experiment examined the direct and transgenerational effects of 10 different wavelength treatments of ALAN on behaviour in zebrafish (Danio rerio), a diurnally active model organism. Across a 10-day period, female zebrafish were exposed to either a monochromatic wavelength, white light ALAN, or to a control treatment, and the individual impacts of each treatment on locomotion and anxiety-like behaviours were examined both for solitary fish and fish in groups. We found the strongest impact at short wavelengths (365 to 470 nm), with individuals and groups of zebrafish showing more anxiety-like behaviour after fewer nights of ALAN exposure relative to the other wavelengths. Furthermore, F1 offspring born from ALAN-exposed mothers displayed less frequent movement and shorter movement distances despite never being exposed to ALAN themselves, regardless of the spectral treatment. Our results highlight both the specific and broad-spectrum potential for ALAN to cause disruption to locomotion in adult zebrafish and their offspring.