The aim of the current study is to evaluate Cu accumulation levels in Lepidium sativum L. growing in different media, as -1 distilled water (DW)with +125 and +250 mg. l added copper ions, and purified industrial wastewater (PIWW), with the same 2+ concentrations of copper ions in addition. It was registered that the accumulation of Cu in Lepidium sativum L. depended on 2+ the concentration of ions in the water media, as the double increase of Cu reflected approximately three times more accumulation 2+ in the tissues of control samples, while in IPWW the effect was only double bioaccumulation. The excess of Cu ions in the media did not influence the accumulation of Mn ions. In media from purified wastewater, the addition of copper ions diminished the uptake of Fe ions and increased Zn bioaccumulation.