The working paper analysed the infrastructure of the Russian-Swedish border from a transcultural perspective. The history of the border was split into three periods following major changes in political border regimes. The first period covered the history of the border between Sweden and the Novgorod Republic after its formal delimitation in 1323. The annexation of the Novgorod Lands to the Grand Duchy of Moscow in 1478 marked the beginning of the second period. The third period, which is discussed in detail, covered the history of border infrastructure between the transition of large part of the Novgorod Lands to Sweden in 1617 and 1700. Departing from the debate whether the border was a line or a zone and overcoming state-centered approaches, the working paper demonstrated that the existence of several parallel border regimes during different periods enabled the simultaneous existence of the border as a line and a zone pertaining to different social interactions and subject to manipulation by authorities. The consolidation of the border did not follow the Treaty of Stolbovo (1617), but owed to local demands and an accidental event of an epidemic in 1629–1630. Following the temporary consolidation of the border, the state established firm border control used for duty collection.