The notion of agency has a long history in the theorization of laboratory work. This study contributes to the conversation about agency by bringing insights from the ways in which scientists interact with the material and biological tools in the genetics laboratory. Qualitative data was obtained through participant observation and interviews in a Drosophila melanogaster (fruit fly) laboratory of North Carolina State University. The focus of this research is the aspects about which researchers are constantly mindful when performing laboratory tasks. The study follows Drosophila’s transformation in the lab from living model organism to DNA/RNA samples to a series of data points, and eventually data analyses. This transformation occurs in three stages, which are not consecutive. In the fly work stage, scientists conduct assays on fruit flies to test their behavior or measure physical traits. In the molecular stage, scientists conduct molecular protocols using flies’ DNA or RNA. In the data analysis stage, researchers conduct statistical analyses, and use GWAS Pipeline to make correlations between the phenotype and genotype of fruit flies. This study emphasizes the ambivalence that the scientists in the Drosophila lab have towards their tools’ agency. It argues for the importance of situating agency in the social community of the laboratory studied. Rheinberger’s concept of instrument-experimental object interface and Knorr-Cetina’s concept of indeterminacy help frame the role of the scientist as primary decision maker in the Drosophila lab. Furthermore, the notion of sociality brings forth the significance of the social context of the laboratory.