Abstract : A variety of statistical procedures was used to analyze the motility parameters of separate populations of hyperactivated and non-hyperactivated rabbit sperm cells. The parameter Wobble (WOB) was the most efficient in classifying hyperactivation. In combination with Curvilinear Velocity (VC), at least 98% of the cells were correctly classified as either hyperactivated or non- hyperactivated. The threshold values for the two motility parameters were specific for the instrument used to measure the motion parameter. The ability to objectively identify and quantify hyperactivated motility is potentially of great use for clinical and toxicological assessment of fertility.