Kidney failure results in a decrease in erythropoietin, leading to the occurrence of anemia. Therefore, EPO therapy is given to increase Hb levels, erythrocyte count and hematocrit values. This study was to determine the difference in Hb levels, erythrocyte count and hematocrit values before and after administration of EPO in patients with kidney failure at Bangil Pasuruan Hospital in January-February 2024 The research design uses comparative analysis with the cross sectional method. Data were obtained from 45 results of examination of Hb levels, erythrocyte count and hematocrit values before and after EPO administration. Then the data was tested using the T dependent test. The significance value of Hb level was 0.021, the number of erythrocytes was 0.036 and the hematocrit value was 0.031. Because the sig value < 0.05, was concluded that there was a significant difference between Hb levels, erythrost count and hematocrit values before and after EPO therapy.