Edited by Ralf J. Sommer. WormMethods editor, Victor Ambros. Last revised June 29, 2006. Published July 17, 2006. This chapter should becited as: Pires da Silva, A. Pristionchus pacificus genetic protocols (July 17, 2006), WormBook, ed. The C. elegans Research Community,WormBook, doi/10.1895/wormbook.1.114.1, http://www.wormbook.org.Copyright: © 2006 Andre Pires da Silva. This is an open-access article distributed under the terms of the Creative Commons AttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.