PDGF upregulates CLEC-2 to induce T regulatory cells
Copyright policy )PDGF upregulates CLEC-2 to induce T regulatory cells
The effect of platelet derived growth factor (PDGF) on immune cells is not elucidated. Here, we demonstrate PDGF inhibited the maturation of human DCs and induced IL-10 secretion. Culture of PDGF-DCs with T cells induced the polarization of T cells towards FoxP3 expressing T regulatory cells that secreted IL-10. Gene expression studies revealed that PDGF induced the expression of C-type lectin like receptor member 2, (CLEC-2) receptor on DCs. Furthermore, DCs transfected with CLEC-2 induced T regulatory cells in DC-T cell co-culture. CLEC-2 is naturally expressed on platelets. Therefore, to confirm whether CLEC-2 is responsible for inducing the T regulatory cells, T cells were cultured with either CLEC-2 expressing platelets or soluble CLEC-2. Both conditions resulted in the induction of regulatory T cells. The generation of T regulatory cells was probably due to the binding of CLEC-2 with its ligand podoplanin on T cells, since crosslinking of podoplanin on the T cells also resulted in the induction of T regulatory cells. These data demonstrate that PDGF upregulates the expression of CLEC-2 on cells to induce T regulatory cells.
- University of California, San Francisco United States
- University of California, Irvine United States
- University of California System United States
- UNIVERSITY OF CALIFORNIA-IRVINE
Blood Platelets, dendritic cell, Cells, T-Lymphocytes, T regulatory cells, Immunology, Oncology and Carcinogenesis, 610, Transfection, T-Lymphocytes, Regulatory, Dose-Response Relationship, Lectins, Paracrine Communication, 2.1 Biological and endogenous factors, Clec-2, Humans, Lectins, C-Type, Immune response, Cells, Cultured, Platelet-Derived Growth Factor, Cultured, Membrane Glycoproteins, Biomedical and Clinical Sciences, C-Type, Dose-Response Relationship, Drug, Inflammatory and immune system, Immunity, Oncology and carcinogenesis, Forkhead Transcription Factors, Dendritic Cells, PDGF, Regulatory, Coculture Techniques, Interleukin-10, Up-Regulation, podoplanin, Phenotype, platelets, Immunology and Microbiology Section, Drug, Signal Transduction
Blood Platelets, dendritic cell, Cells, T-Lymphocytes, T regulatory cells, Immunology, Oncology and Carcinogenesis, 610, Transfection, T-Lymphocytes, Regulatory, Dose-Response Relationship, Lectins, Paracrine Communication, 2.1 Biological and endogenous factors, Clec-2, Humans, Lectins, C-Type, Immune response, Cells, Cultured, Platelet-Derived Growth Factor, Cultured, Membrane Glycoproteins, Biomedical and Clinical Sciences, C-Type, Dose-Response Relationship, Drug, Inflammatory and immune system, Immunity, Oncology and carcinogenesis, Forkhead Transcription Factors, Dendritic Cells, PDGF, Regulatory, Coculture Techniques, Interleukin-10, Up-Regulation, podoplanin, Phenotype, platelets, Immunology and Microbiology Section, Drug, Signal Transduction
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