Given the pharmacological importance of tropane alkaloids and the possibilityof producing them from plant tissue cultures of plant species such as Brugmansia candida, the main goal of the present paper was to evaluate the alkaloid production of root cultures developed ina bioreactor. We set up a bioreaction system consisting of two units, one forbiomass growth and the other one for liquid medium conditioning. After 21 days of culture, biomass and scopolamine production were evaluated, resulting in the extraction of 9.5 mg of scopolamine/g of dry roots. This value was higher than the one obtained in a previous culture in Erlenmeyer flasks (6mg/g of dry roots) and higher than the scopolamine extracted from hairy roots cultures using a bioreactor reported in other studies. Regarding biomass, the growth index (GI) was 1.58, similar to the GI obtained with cultures in Erlenmeyer flasks (GI 1.4), which suggests that scaling up the process did not affect biomass productionnegatively.