Research with the objective of finding efficient (non residual) sterilants for the purpose of greater automatization in establishing aseptic in vitro tissue culture was conducted on cherry rootstocks CAB 6P and Gisela 6. Two ways of sterilization were examined (NaOCl and ozone O3) through survival rate on three types of explants; buds with primordial leaves, buds without primordial leaves and nodal non lignified segment with axillary buds. Ozone resulted with the high rate of survival (from 57 to 93%) regardless of the type of explants and the rootstock variety. By introducing a complete bud without destruction significantly resulted with the higher rate of survival (from 90 to 97%). Results indicate the potential for ozone sterilization of the starting material not only as an ostensible but the perforated (sub-cuticular transpiration) agent.

Istraživanja s ciljem pronalaženja učinkovitih sterilizanata, u svrhu veće automatizacije u uspostavi aseptične kulture tkiva in vitro, provedena su na podlogama trešnje CAB 6P i Gisela 6. Ispitivana su dva načina sterilizacije (NaOCl i ozon O3), prateći stopu preživljavanja na tri tipa eksplantata; pupovi s primordijalnim lišćem, pupovi bez primordijalnoga lišća i nodijalni nelignificirani segment s aksilarnim pupom. Ozon je rezultirao visokom stopom preživljavanja (od 57 do 93%), bez obzira na vrstu eksplantata i varijante podloge. Uvođenjem cjelovitoga pupa bez destrukcije značajno je rezultiralo višom stopom preživljavanja (od 90 do 97%). Rezultati ukazuju na potencijal sterilizacije ozonom inicijalnoga biljnoga materijala, ne samo kao površinskoga, nego i penetrirajućeg (subkutikularnog) agenta.