The unicellular eukaryote S. cerevisiae represents an invaluable resource for the study of basic eukaryotic cellular and molecular processes. The combination of a high genetic amenability, numerous genetic tools, and vast genomic resources makes it one of the most versatile model organisms, employed in a wide range of basic research disciplines. However, its small size compared to other eukaryotic organisms has limited its use for the study of sub-cellular structures. The diameter of unbudded yeast cells ranges approximately between 4 μm in haploids and 6 μm in diploids (Milo and Phillips 2015), complicating conventional diffraction-limited light microscopy approaches.