Whitefly Trialeurodes vaporariorum Westwood is an important pest and significant limiting factor for glasshouse production of vegetables in regions with moderate climate. Over the past several decades, its control has been increasingly based on the use of the chalcidoidea parasitoid Encarsia formosa Gahan. However, satisfactory results have not always been achieved by using this wasp, which is why further investigation of the biological characteristics of the whitefly and the autochthonous species of its natural enemies (parasitoids and predators) has been carried out. In Serbia, over 3000 ha of glasshouses and plastic greenhouses are used in vegetable production. In this investigation, we identified four species of parasitoids wasps (family Aphelinidae) and 15 predatory species (families Miridae, Nabidae, Anthocoridae, Coccinellidae and Chrysopidae) in several different parts of Serbia. The registered species of chalcidid wasps from the genus Encarsia were: Encarsia formosa Gahan, Encarsia tricolor Foerster, Encarsia partenopea Masi and Encarsia lutea Masi. The most important predatoryspecies were: Clitostethus arcuatus Rossi, Synharmonia conglobata Linnaeus, Adalia bipunctata Linnaeus, Propylea quatuordecimpunctata Linnaeus, Tytthaspis sedecimpunctata Linnaeus, Hippodamia tredecimpunctata Linnaeus, Adonia variegata Goeze, Chrysopa carnea Stephen, Crhysopa phyllochroma Wesmael, Nabis ferus Linnaeus, Nabis pseudoferus Remane, Nabis brevis Scholtz and Dicyphus errans Wolff.