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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Research@WURarrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Research@WUR
Conference object . 1997
Data sources: Research@WUR
Acta Horticulturae
Article . 1997 . Peer-reviewed
Data sources: Crossref
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SOMATIC EMBRYOGENESIS IN TULIP

Authors: Gude, H.; Dijkema, M.H.G.E.;

SOMATIC EMBRYOGENESIS IN TULIP

Abstract

Because the natural propagation rate of tulip is low, modern breeding requires a rapid in vitro propagation method. The best method available at the moment, adventitious shoot formation on stem explants, has a low propagation factor and is laborious, time-consuming and hence expensive. It is the long term aim of the present study to develop a propagation method, consisting of a cell suspension phase, and a regeneration phase, during which the cell clusters regenerate through somatic embryogenesis. A system like this would enable the rapid production of large numbers of bulblets at low costs. In order to obtain friable callus for the start of a cell suspension, bulb scale and stem explants were cultured on a nutrient medium containing the auxins 2,4-D or Picloram at a concentration range from 0.5-50 μM. Undifferentiated and 'meristematic' type of callus on bulb scale explants an were induced. The meristematic type seemed the most suitable to start a liquid culture. In liquid medium the meristematic nodules divided into smaller units, which could be released from the explant by shaking. On stem explants on medium with 5 or 50 μM 2,4-D or Picloram somatic embryos were formed. Morphological and anatomical data on the development of these somatic embryos are presented. The ability to produce somatic embryos offers us the opportunity to use embryo-tissue for the production of embryogenic callus.

Country
Netherlands
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Keywords

Life Science

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
15
Average
Top 10%
Average
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