To improve the sensitivity and throughput of molecular testing for rabbit caliciviruses we have developed a multiplex TaqMan RT-qPCR assay that detects the three different pathogenic rabbit calicivirus capsid genotypes currently circulating in Australia. Target sequences for all three variants (GI.1c, GI.2, GI.1a) are located within the VP60 capsid sequence. This will also detect subgenomic RNAs, increasing the sensitivity of the method when compared to assays targeting the non-structural gene sequences. Currently this assay is designed to be run as a one-step RT-qPCR assay for high throughput, rapid diagnostic purposes. It has not been designed for quantification of virus loads. For that purpose, the universal lagovirus SYBR-green assay is still recommended (Hall et al, 2018). This assay has not been designed for the detection of recombinant viruses, and will only return the capsid variant/s present in a sample.