
Summary: Procedure used to measure the concentration of Blood Urea Nitrogen(BUN) in blood, plasma, and serum. Urea is determined by the enzymatically coupled reactions of urease (to form ammonia) and glutamate dehydrogenase (conversion of ammonia and glutamate to glutamine with oxidation of NADH to NAD). The rate of NAD formation is monitored by the change in absorbance at 340 nm.
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